ABSTRACT
Objective:To establish an ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for plasma caffeine concentration detection, and to explore the clinical value of caffeine therapeutic drug monitoring (TDM) in the treatment of premature infants with respiratory distress syndrome (RDS).Method:Take the plasma sample in a centrifuge tube, add the caffeine deuterated isotope internal standard, then add the protein precipitant, vortex the mixture thoroughly, and centrifuge the supernatant to enter the mass spectrometry analysis. The mobile phase were methanol and water, gradient elution; the column temperature was 45 ℃, the method was established using Shimadzu LC-30AD-CL liquid system and AB SCIEX 4500 QTRAP mass spectrometer, and the sensitivity, specificity, linearity, accuracy imprecision, matrix effect, and carry-over of the method were evaluated. Sample from 30 patients diagnosed with neonatal RSD were collected in the Department of Neonatology of Renmin Hospital of Wuhan University from February to April 2021, then detected the trough concentration of caffeine in premature infants with RDS after taking the same dose of caffeine to assess the impact of individual variation on caffeine drug concentration.Results:The detection limit of caffeine was 0.02 μg/ml, and the lowest limit of quantification was 0.05 μg/ml. It showed good linearity ( R2=0.9986, R>0.99) in the concentration range from 1.0 to 100.0 μg/ml, specificity (recovery rate of 85.52%-114.12%), accuracy (recovery rate 85.97%-114.53%), intra-day and inter-day imprecision ( CV 6.01%-11.28%), matrix effects and carryover pollution were negligible. The trough concentration of 30 preterm infants with RSD after taking the same dose of caffeine (10 mg/kg) was (25.45±11.61) μg/ml, and the coefficient of variation was 44.88%. Conclusion:This study established an accurate and reliable UPLC-MS/MS method with low sample consumption to monitor the blood concentration of caffeine; caffeine TDM has certain clinical application value, which can be used to assist RDS diagnosis and treatment and improve the efficacy of caffeine.
ABSTRACT
Objective@#To understand home-based physical exercise of primary and secondary school students in the main urban area of Chongqing during the peviod of coronarirus outbreak, and to provide reference for the rapid development of physical health and development of primary and secondary school students.@*Methods@#A network questionnaire survey of 4 200 students in 14 primary and secondary schools in 7 districts of Chongqing City were selected by using stratified clustering method.@*Results@#During the epidemic outbreak,for primary and secondary school students in the main urban area of Chongqing, the weekly online physical education courses of students accounted for 57.97% of the total poorticipants, daily eye exercise students accounted for 81.47%, weekly home exercise of more than 3 times accounted for 22.50%, physical exercise lasting for more than 1 h accounted for 14.47% which differed by gender and study period(χ2=51.48, 17.03, 38.85, 29.52; 618.74, 132.14, 187.26, 54.10, P<0.05). About 50.11% of students have mastered 3 or more sports skills, 83.09% of students participate in online physical fitness study; Which differed in gender and study period(χ2=11.83, 6.25; 172.15, 228.51, P<0.05). 34.45% of parents in home physical exercise support accompanying students to participate in home exercise, 45.95% of parents support students to participate in home exercise but not accompany the exercise, 14.95% of parents attitude is not clear, 4.65% of parents do not support students to participate in home exercise, 14.93% of families set up home exercise program,all of which differed significantly by gender and study period(χ2=43.12, 228.35, P<0.01).@*Conclusion@#Home-based physical exercise behavior is low during the coronavirus outbreak in Chongqing primary and secondary school students, and it is necessary to increase the cooperative education of home schools to promote the formation of students’ physical exercise habits.
ABSTRACT
Serum lipid peroxides [LPO] was estimated in 39 normal subjects and equal number of patients of nasopharyngeal carcinoma [NPC] by thiobarbital acid [TEA] colorimeric method. The LPO level of NPC group was significantly higher [P < 0.01] than in normal patients. It appears that there is a close relationship between LPO and NPC. The raised LPO may be helpful in the dignosis of NPC